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KMID : 1007520200290010035
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Food Science and Biotechnology
2020 Volume.29 No. 1 p.35 ~ p.43
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Protein purification from Arachis hypogaea in one step: stability studies and anticarcinogenic analysis
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Ahmad Afaque
Verma Hirday N.
Bharti Prahalad
Pandey Kamlesh
Khan Shahbaz
Dev Kapil
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Abstract
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The study involved purification of trypsin inhibitor from the seeds of Indian peanuts (Arachis hypogaea), a member of leguminosae family. The inhibitor was purified to homogeneity via three sequential step procedure i.e., salt precipitation to anion-exchange chromatography. The purity and molecular mass was detected using SDS PAGE analysis i.e.?~?16 kDa. The purified inhibitor termed as Peanut Trypsin Inhibitor (PTI) which inhibits trypsin belonging to serpins family. Anti- neoplastic potential on breast cancer cells (MCF-7) and normal Human Embryonic Kidney cells (HEK) was determined using MTT assay. PTI exhibited IC50 value of?~?18.412 μg/mL in HEK cells compared to?~?9.635 μg/mL in MCF-7 cells. The values were quite comparable to curcumin, the standard anticancer drug demonstrating IC50 values of?~?21.581 μg/mL and?~?7.135 μg/mL in HEK and MCF-7 respectively. Therefore, we conclude that PTI may be used as supplement along with the conventional drugs for increased efficacy in the treatment of cancer.
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KEYWORD
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Protease inhibitor, Trypsin inhibitor, Arachis hypogaea, Anticancer, Peanut
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